I was recently asked to post a couple of guidelines for ferret
autopsies. No, this is not going to be a primer on how to autopsy your own
ferret, but some guidelines on how to maximize the diagnostic information
which you can get from a post mortem. Many vets actually do not know much
about doing autopsies, so this information may be very important to you.
1. Have the post done as quickly as possible. From the moment of
death, a ferret's body begins to decompose. The different tissues decompose
at different rates, based on their composition. As a general rule, the
digestive system, by its very nature, decomposes fastest - you might say it
digests itself. The gall bladder which is filled with bile, a digestive
compound, goes first - usually within 2 hours. Between six and twelve
hours, the lining of the digestive tract, what I usually rely on to make a
diagnosis of ECE, is gone, due to the presence of bacteria, food, and
digestive enzymes. The pancreas, a storehouse of digestive enzymes, also
decomposes fast. Generally, if you have an undiagnosed digestive disease
and the tissues aren't in formalin within 12 hours, you have markedly
diminished your chances of making a diagnosis.
2. The end point of decomposition is when the tissues are placed
in formalin, or another fixative, by the vet. Formalin immediately halts
all decomposition by cross-linking proteins. What I see under the
microscope is a picture of the tissue when it hit the formalin.
3. Refrigeration slows decomposition, but does not stop it.
Freezing damages the tissues beyond all recognition under the microscope -
all the water in the cells is frozen into ice crystals, and when they are
thawed, the cells are destroyed. Never freeze tissues that are going to
pathologists for microscopic examination. Freezing is only for tissues to
preserve pathogens for later culture.
4. The ideal way to obtain a post mortem is immediately following
euthanasia. Have your vet block out time to euthanize and do the post.
5. If you are doing a necropsy - take the widest range of tissues
possible - even tissues you don't think are involved. One of the most
frustrating things about being a pathologist is only getting two or three
tissues in an animals in which a disease was not diagnosed ante mortem. If
you are not sure what is going on, sample everything.
6. Don't due an "abdominal post" - sample the thoracic
organs, as well as a range of lymph nodes, eyes, and the nervous system.
7. If the animal was showing neurological signs, it is very
important to submit brain and spinal cord.
8. Make sure to use 10 parts formalin to 1 part tissue for proper
fixation. Throwing a large lump of tissue into a small amount of formalin
delays fixation and allows the tissue to continue to decompose.
9. This is most important - if you don't post within 48 hours -
you probably have missed the window of opportunity. The amount of
diagnostic information that is lost after 48 hours probably will render an
autopsy into a frustrating and cost-ineffective exercise for the owner,
veterinarian, and pathologist.
10. Remember that animals die at the least opportune moment -
usually 5 minutes after the vet has closed the clinic for the weekend. If
you have a sick ferret that may not make it, and you want a necropsy done,
make your wishes known to your vet before the moment comes. They will more
likely be available after hours for you.
Just a couple of guidelines which may save you extra grief during at a
difficult time.
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